Effect of Excess Gravitational Force on Cultured Myotubes in Vitro
Shigehiro Hashimoto, Haruka Hino, Takeshi Iwagawa
An effect of an excess gravitational force on cultured
myoblasts has been studied in an experimental system with
centrifugal force in vitro. Mouse myoblasts (C2C12) were
seeded on a culture dish of 35 mm diameter, and cultured in the
Dulbecco’s Modified Eagle’s Medium until the sub-confluent
condition. To apply the excess gravitational force on the
cultured cells, the dish was set in a conventional centrifugal
machine. Constant gravitational force was applied to the
cultured cells for three hours. Variations were made on the
gravitational force (6 G, 10 G, 100 G, 500 G, and 800 G) with
control of the rotational speed of the rotator in the centrifugal
machine. Morphology of the cells was observed with a phasecontrast
microscope for eight days. The experimental results
show that the myotube thickens day by day after the exposure
to the excess gravitational force field. The results also show
that the higher excess gravitational force thickens myotubes.
The microscopic study shows that myotubes thicken with
fusion each other. Full Text
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