Effect of Ultrasonic Vibration on Proliferation and Differentiation of Cells
Haruka Hino, Shigehiro Hashimoto, Yusuke Takahashi, Hiroaki Nakajima
The effect of mechanical stimulation of vibration on proliferation and differentiation of cells has been studied in vitro. To apply the vibration on the cells, a piezoelectric element was attached on the outside surface of the bottom of the culture plate of six wells. The piezoelectric element was vibrated by sinusoidally alternating voltage at 1.0 MHz generated by a function generator. Five kinds of cells were used in the experiment: C2C12 (mouse myoblast cell), L929 (fibroblast connective tissue of mouse), Hepa1-6 (mouse hepatoma cell), HUVEC (human umbilical vein endothelial cell), and Neuro-2a (mouse neural crest-derived cell line). After the incubation for 24 hours, cells were exposed to the ultrasonic vibration intermittently for three days: for thirty minutes per day. At the end of the experiment, the number of cells was counted by colorimetric method with a microplate photometer. In the case of Neuro-2a, the total length of the neurite was calculated at the microscopic image. The experimental study shows following results. Cells are exfoliated by the strong vibration. Proliferation and differentiation of cells are accelerated with mild vibration. The optimum intensity of vibration depends on the kind of cells.