The University of Dar Es Salaam has developed the web-based laboratory for Time and Frequency Response Analysis. The purpose of this web-based laboratory is the utilization of real data from real experiments, in terms of instrumentation and experimental circuits, rather than simulations. The use of webbased laboratory came after realizing the difficulties imposed by the traditional laboratories. Web-based laboratories allow students and educators to interact with real laboratory equipment located anywhere in the world at anytime. This paper presents the implementation of web-based laboratory of single stage common emitter, resistor capacitor coupled amplifier using National Instruments Educational Laboratory Virtual Instrument Suite platform. Two components are deployed: time response analysis and frequency response analysis. The experiment allows students to carryout time and frequency analysis of the amplifier. The modular can be used to any microelectronic circuits to carry out any time response and frequency response analysis. Both the time response and frequency response analysis results of the amplifier are validated.