Orientation of Cells Cultured in Vortex Flow with Swinging Plate in Vitro
Shigehiro Hashimoto, Masahide Okada
An effect of flow on cell culture has been studied in vitro. A silicone disk was placed in the center of culture dish of 52 mm internal diameter to make a doughnut-shaped canal. The dish was placed on a tilted plate, which rotates to make a vortex flow around the silicone disk with a swing motion. Variations were made on the diameter (20 mm, 30 mm, and 40 mm) of the silicone disk and the rotational speed (2.1 rad/sec, 5.2 rad/sec) of the swinging plate, which tilts with 0.1 rad from the horizontal plane. Five kinds of cells were cultured in the vortex flow of Dulbecco’s Modified Eagle’s Medium for seven days: C2C12 (mouse myoblast), L6 (rat skeletal muscle cell), A7r5 (rat aortic smooth muscle cell), CS-2P2-C75 (primary normal porcine aortic endothelial cell), and L929 (mouse fibroblast). The experiments show the following results. The orientation of cells depends on flow and on kinds of cells. A7r5 and CS-2P2-C75 line along the streamline of the flow. C2C12 and L6 adhere along the direction of the flow in the first stage, and tilt to the perpendicular direction to the flow differentiating to myotubes with fusion in the second stage.