The behavior of each cell on the micro ridge pattern after the continuous stimulation by a tangential force has been studied in vitro. The stripe-pattern (0.7 μm height, 3 μm width, and 3 μm interval) was made on the surface of the scaffold plate to control the orientation of each cell during the force field stimulation. Variation was made on the angle between the longitudinal direction of the ridge and the direction of the tangential force: 0 degrees, 45 degrees, and 90 degrees. Myoblast (C2C12: mouse myoblast cell line) was used in the experiment. To apply the tangential force field to the cells, the scaffold plate was set in the tube in a conventional centrifugal machine placed in an incubator. After centrifugation for 5 hours, the behavior of each cell at the time-lapse microscope images was traced for 10 hours. The experimental results show that cells on the ridge-lines oriented perpendicular to the tangential force field have the higher activity (migration, and deformation) after stimulation.