Journal of
Systemics, Cybernetics and Informatics
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ISSN: 1690-4524 (Online)


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Editorial Advisory Board's Chair
William Lesso

Editor-in-Chief
Nagib C. Callaos


Sponsored by
The International Institute of
Informatics and Systemics

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Are We Meeting Pedagogic Requirements? – The Quadratic Equation
Russell Jay Hendel
(pages: 1-7)

Facilitating Effective Student Participation in an Online Environment
Nanda van der Stap, Risa Blair
(pages: 8-11)

Virtual Global Classrooms without Walls: Collaborative Opportunities for Higher Learning Engagement
Cathy MacDonald, Debra Sheppard-LeMoine
(pages: 12-16)

Augmented Reality as Visual Communication for People with ASD
Esteban Menéndez, María Daniela López de Luise
(pages: 17-21)

Study of Race Condition: A Privilege Escalation Vulnerability
Tanjila Farah, Rashed Shelim, Moniruz Zaman, Delwar Alam
(pages: 22-26)

From the Lab to the Field: 3D Technology Supporting Study and Conservation Processes on Ancient Egyptian Artefacts
Paola Buscaglia, Elena Biondi, Alessandro Bovero, Tomasso Quirino
(pages: 27-32)

Digital Forensics Compute Cluster (DFORC2) – A New High Speed Distributed Computing Capability for Digital Forensics
Daniel Gonzales, Zev Winkelman, Trung Tran, Ricardo Sanchez, Dulani Woods, John Hollywood
(pages: 33-38)

Proposal of a Bus Location System Based on Participatory Sensing with BLE Devices and Smartphones
Katsuhiro Naito, Katsuyuki Tanaka
(pages: 39-44)

Technical Change and Employment in an Emerging Economy
Humberto Merritt
(pages: 45-53)

Interpretation of the Results of a Case Study about Impacts and Influences of Exogenous Variables in the Planning of Chronogram and Budget in Software Projects
Altino José Mentzingen de Moraes
(pages: 54-59)

Flipped Classroom – A Flexible Way of Teaching Technology Usage for Diagnostics in the Medical Subdomain ENT
Walter Koch, Jochen Schachenreiter, Klaus Vogt, Gerda Koch
(pages: 60-64)

ERP Selection: The Lifeblood of an Organization
Desmond (Tres) Bishop
(pages: 65-69)

Proposing an Education System to Judge the Necessity of Nuclear Power in Japan
Ariyoshi Kusumi
(pages: 70-74)

Biometric Encryption System for Increased Security
Ranjith Jayapal, Pramod Govindan
(pages: 75-80)

BIM as a Structural Safety Study Tool in Case of Fire - BIMSCIP
Marcelo Franco Porto, José Ricardo Queiroz Franco, Luiza Giori Barcellos Correa, Lucas Vinicius Ribeiro Alves, Renata Maria Abrantes Baracho
(pages: 81-86)

Evaluating the Construct Validity of Basic Science Curriculum Assessment Instrument for Critical Thinking: A Case-Study
Chau-Kuang Chen, Adriana Marie Horner, Michelle Scott, Stephanie C. McClure
(pages: 87-92)

The Outer Banks Study – Physio-Chemical Parameters for Water Quality Testing/Professional Development Program for Teachers
Joseph Stringer, Timothy Bowman, Keith Vinson, Catherine Warnecke, Nora Lewis, William Slattery, Suzanne K. Lunsford
(pages: 93-97)


 

Abstracts

 


ABSTRACT


Comparative Infectomic Analysis and Molecular Characterization of CglE, the Invasin IbeA Homologous Protein, Which Contributes to the Pathogenesis of Meningitic E. Coli K1 Infection

Hong Cao, Lidan Chen, Jun Yang, Shuji Gong, Hao Zhou, Yong Jiang, Sheng-He Huang


CglE is a putative dihydrolipoamide dehydrogenase (DLDH) that shares significant protein sequence homology (50% identity and 70% similarity) to the IbeA invasin contributing to the pathogenesis of E. coli meningitis. However, the biochemical, biological and pathogenic functions of CglE are unknown. In order to characterize the role of CglE in the pathogenesis of meningitic infections, infectomic, bioinformatics and molecular approaches were used to analyze and predict its structure and function. First, our comparative infectomic studies showed that CglE and IbeA are present in the genetic island GimA as a pair of homologous proteins that are encoded by two different operons, cgl (GimA2) and ibe (GimA4), at different locations. A similar pair of proteins is also present in Silicibacter sp which belongs to the most abundant and ecologically relevant marine bacterial groups. Meningitic E. coli K1 and Silicibacter sp have to survive under harsh environments (cerebrospinal fluid and ocean) with poor nutrition, suggesting that this pair of proteins is important for energy metabolism in the both microbes. Secondly, bioinformatic analysis indicated that CglE is a putative DLDH, which is the E3 component of pyruvate dehydrogenase complex. A FAD-binding domain and homologous flavoprotein regions are present in CglE. DLDH has been identified as virulence factors contributing to the pathogenesis of hepatitis C virus and pneumococcal infections. The sequence of CglE is homology to that of IbeA, an invasion protein of meningitic E. coli, and the surface probability and hydrophilicity are very similar to each other. Thirdly, the expression, purification and biochemical analysis of CglE protein was further carried out to determine whether CglE shares similar functions as IbeA and whether it is a new class of DLDH. The cglE gene was amplified by PCR and subcloned into pET22b(+) then expressed in E. coli BL21(DE3) as a fusion protein with His6 tag at its C-terminus induced by IPTG. CglE fusion protein was purified. Like IbeA, CglE is able to bind to an IbeA-binding protein, vimentin. In further studies, we will examine whether CglE is a new class of DLDH and how it contributes to the pathogenesis of meningitic infections.

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