Journal of
Systemics, Cybernetics and Informatics
HOME  |   CURRENT ISSUE   |   PAST ISSUES   |   RELATED PUBLICATIONS     IIIS   |   SEARCH     CONTACT US
 



ISSN: 1690-4524 (Online)


Indexed by
EBSCO, Cabell, DOAJ (Directory of Open Access Journals)Benefits of supplying DOAJ with metadata:
  • DOAJ's statistics show more than 900 000 page views and 300 000 unique visitors a month to DOAJ from all over the world.
  • Many aggregators, databases, libraries, publishers and search portals collect our free metadata and include it in their products. Examples are Scopus, Serial Solutions and EBSCO.
  • DOAJ is OAI compliant and once an article is in DOAJ, it is automatically harvestable.
  • DOAJ is OpenURL compliant and once an article is in DOAJ, it is automatically linkable.
  • Over 95% of the DOAJ Publisher community said that DOAJ is important for increasing their journal's visibility.
  • DOAJ is often cited as a source of quality, open access journals in research and scholarly publishing circles.
JSCI Supplies DOAJ with Meta Data
, Academic Journals Database, and Google Scholar


Listed in
Cabell Directory of Publishing Opportunities and in Ulrich’s Periodical Directory


Re-Published in
Academia.edu
(A Community of about 40.000.000 Academics)


Editorial Advisory Board's Chair
William Lesso

Editor-in-Chief
Nagib C. Callaos


Sponsored by
The International Institute of
Informatics and Systemics

www.iiis.org
 

Editorial Advisory Board

Editors

Journal's Reviewers
 

Description and Aims

Submission of Articles

Areas and Subareas

Information to Contributors

Editorial Peer Review Methodology

Integrating Reviewing Processes


Effect of Flow on Cultured Cell at Micro-Pattern of Ridge Lines
Haruka Hino, Shigehiro Hashimoto, Yusuke Shinozaki, Hiromi Sugimoto, Yusuke Takahashi
(pages: 1-7)

Bridging the Semantic and Lexical Webs: Concept-Validating and Hypothesis-Exploring Ontologies for the Nexus-PORTAL-DOORS System
Adam Craig, Seung-Ho Bae, Carl Taswell
(pages: 8-13)

The Learning Science through Theatre Initiative in the Context of Responsible Research and Innovation
Zacharoula Smyrnaiou, Elena Georgakopoulou, Menelaos Sotiriou, Sofoklis Sotiriou
(pages: 14-22)

Perceptions and Preferences of High School Students in STEM: A Case Study in Connecticut and Mississippi
Bin (Brenda) Zhou, Clifford Anderson, Feng Wang, Lin Li
(pages: 23-26)

A Study on the Meaning of the ‘Lifelong Learning to Be’ Implicated in the Philosophy of Nietzsche
Kwanchun Lee, Soo Yeon Choi, Un Shil Choi
(pages: 27-32)

Barriers to Social Innovation and Ways of Overcoming them in Latvia
Karine Oganisjana, Yuliya Eremina, Salome Gvatua, Benjamin Ngongo Kabwende, Ozoemena Joseph Chukwu
(pages: 33-38)

Unmanned Aerial Vehicle-Based Automobile License Plate Recognition System for Institutional Parking Lots
Julian Dasilva, Ricardo Jiménez, Roland Schiller, Sanja Zivanovic González
(pages: 39-43)

Dialectal Atlas of the Arab World - between Intention and Reality
Oleg Redkin, Olga Bernikova
(pages: 44-47)

IT Risk and Chaos Theory: Effect on the Performance of South African SMEs
Anass Bayaga, Stephen Flowerday, Liezel Cilliers
(pages: 48-53)

How to Apply the User Profile Usability Technique in the User Modelling Activity for an Adaptive Food Recommendation System for People on Special Diets
Lucrecia Llerena, Nancy Rodríguez, Pablo Gómez-Abajo, John W. Castro
(pages: 54-63)

Policies, Legislation and Regulatory Compliance Governance Impact on Strategic Management of Higher Education and Research Institutions in Latvia
Anita Straujuma, Inga Lapina, Elina Gaile-Sarkane, Modris Ozolins
(pages: 64-69)

Parallel Prediction of Stock Volatility
Priscilla Jenq, John Jenq
(pages: 70-73)

Hacking a Bridge: An Exploratory Study of Compliance-Based Information Security Management in Banking Organization
Tesleem Fagade, Theo Tryfonas
(pages: 74-80)

Improvement in the Physical and Psychological Well-Being of Persons with Spinal Cord Injuries by Means of Powered Wheelchairs Driven by Dual Power Wheels and Mobile Technologies
Yee-Pien Yang, Li-Jen Weng, Ye-Yu Yeh, Hui-Fen Mao, Ray-I. Chang
(pages: 81-87)

Data Mediation with Enterprise Level Security
Kevin E. Foltz, William R. Simpson
(pages: 88-93)


 

Abstracts

 


ABSTRACT


Comparative Infectomic Analysis and Molecular Characterization of CglE, the Invasin IbeA Homologous Protein, Which Contributes to the Pathogenesis of Meningitic E. Coli K1 Infection

Hong Cao, Lidan Chen, Jun Yang, Shuji Gong, Hao Zhou, Yong Jiang, Sheng-He Huang


CglE is a putative dihydrolipoamide dehydrogenase (DLDH) that shares significant protein sequence homology (50% identity and 70% similarity) to the IbeA invasin contributing to the pathogenesis of E. coli meningitis. However, the biochemical, biological and pathogenic functions of CglE are unknown. In order to characterize the role of CglE in the pathogenesis of meningitic infections, infectomic, bioinformatics and molecular approaches were used to analyze and predict its structure and function. First, our comparative infectomic studies showed that CglE and IbeA are present in the genetic island GimA as a pair of homologous proteins that are encoded by two different operons, cgl (GimA2) and ibe (GimA4), at different locations. A similar pair of proteins is also present in Silicibacter sp which belongs to the most abundant and ecologically relevant marine bacterial groups. Meningitic E. coli K1 and Silicibacter sp have to survive under harsh environments (cerebrospinal fluid and ocean) with poor nutrition, suggesting that this pair of proteins is important for energy metabolism in the both microbes. Secondly, bioinformatic analysis indicated that CglE is a putative DLDH, which is the E3 component of pyruvate dehydrogenase complex. A FAD-binding domain and homologous flavoprotein regions are present in CglE. DLDH has been identified as virulence factors contributing to the pathogenesis of hepatitis C virus and pneumococcal infections. The sequence of CglE is homology to that of IbeA, an invasion protein of meningitic E. coli, and the surface probability and hydrophilicity are very similar to each other. Thirdly, the expression, purification and biochemical analysis of CglE protein was further carried out to determine whether CglE shares similar functions as IbeA and whether it is a new class of DLDH. The cglE gene was amplified by PCR and subcloned into pET22b(+) then expressed in E. coli BL21(DE3) as a fusion protein with His6 tag at its C-terminus induced by IPTG. CglE fusion protein was purified. Like IbeA, CglE is able to bind to an IbeA-binding protein, vimentin. In further studies, we will examine whether CglE is a new class of DLDH and how it contributes to the pathogenesis of meningitic infections.

Full Text